Chloramphenicol acetyltransferase should not provide methanogens with resistance to chloramphenicol

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منابع مشابه

Chloramphenicol acetyltransferase should not provide methanogens with resistance to chloramphenicol.

Growth of the four methanogens investigated was inhibited by chloramphenicol-3-acetate; therefore, introduction of chloramphenicol acetyltransferase-encoding genes should not confer chloramphenicol resistance on these methanogens. Reduction of the aryl nitro group of chloramphenicol produced a compound which did not inhibit the growth of these methanogens.

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Resistance to chloramphenicol in Proteus mirabilis by expression of a chromosomal gene for chloramphenicol acetyltransferase.

Proteus mirabilis PM13 is a well-characterized chloramphenicol-sensitive isolate which spontaneously gives rise to resistant colonies on solid media containing chloramphenicol (50 micrograms ml-1) at a plating efficiency of 10(-4) to 10(-5). Such chloramphenicol-resistant colonies exhibit a novel phenotype with respect to chloramphenicol resistance. When a single colony grown on chloramphenicol...

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Tinkering with antibiotic resistance: chloramphenicol acetyltransferase and its substrates.

Chloraniphcnicol acctyltransfcrase ( C A T ) ( E C 7.3.1.78) catalyses the 0-acetylation and inactivation of the antibiotic, using acetyl-CoA a s the acyl donor. T h e structural gene for CAT in chlor~rmphenicol-resistant bacteria may be chromosomal. hut in most genera has been observed t o be plasmidborne. All known variants have similar subunit molecular masses ( 75 k l h ) and a high degree ...

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Chloramphenicol Acetyltransferase Variants in Staphylococci

Four electrophoretic variants of chloramphenicol acetyltransferase (types A, B, C and D) found in chloramphenicol-resistant staphylococci were purified by affinity chromatography. Michaelis constants and the kinetics of inactivation with a variety of reagents for the four variants are virtually identical. Their similar amino acid compositions and near identical N-terminal sequences suggest a hi...

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Characterization of chloramphenicol acetyltransferase from chloramphenicol-resistant Staphylococcus aureus.

Chloramphenicol-resistant strains of Staphylococcus aureus contain an inducible enzyme which inactivates chloramphenicol by acetylation in the presence of acetyl coenzyme A. The products of acetylation are chromatographically indistinguishable from those obtained with chloramphenicol-resistant Escherichia coli harboring an R factor. The kinetics of induction of chloramphenicol acetyltransferase...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1984

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.47.4.868-869.1984